NODAL Polyclonal antibody

NODAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 27430-1-AP

Synonyms

nodal homolog (mouse), Nodal homolog

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Validation Data Gallery

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  WB analysis of HeLa using 27430-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27430-1-AP (NODAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27430-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293T using 27430-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human NODAL (27430-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27430-1-AP targets NODAL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NODAL fusion protein Ag26729
• Full Name: nodal homolog (mouse)
• Calculated Molecular Weight: 347 aa, 40 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC104976
• Gene Symbol: NODAL
• Gene ID (NCBI): 4838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nodal is a member of the transforming growth factor-β (TGF-β) superfamily that plays critical roles during embryogenesis. Nodal also regulates cell proliferation, apoptosis, and invasion in cancer cells. However, it appears to exert both tumor-suppressing and tumor-promoting effects, depending on the cell type (PMID: 22645523). Nodal signals through ALK7 receptor to inhibit proliferation and to induce apoptosis in ovarian cancer cell lines and breast cancer cell lines (PMID: 15531507, PMID: 21383881).