NNT Monoclonal antibody, PBS Only

NNT Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F5H6

Cat no : 68194-1-PBS

Synonyms

NNT

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Validation Data Gallery

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  WB analysis of LNCaP using 68194-1-Ig (same clone as 68194-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

  Various lysates (mouse heart tissue and mouse liver tissue were dissected from BALB/c mice) were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 68194-1-Ig (same clone as 68194-1-PBS)

  IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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Product Information

68194-1-PBS targets NNT in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NNT fusion protein Ag4259
• Full Name: nicotinamide nucleotide transhydrogenase
• Calculated Molecular Weight: 1085 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC032370
• Gene Symbol: NNT
• Gene ID (NCBI): 23530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).