NNT Monoclonal antibody

NNT Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F5H6

Cat no : 68194-1-Ig

Synonyms

NNT



Tested Applications

Positive WB detected inpig heart tissue, LNCaP cells, HeLa cells, Jurkat cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat liver tissue, mouse liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68194-1-Ig targets NNT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NNT fusion protein Ag4259
Full Name nicotinamide nucleotide transhydrogenase
Calculated Molecular Weight 1085 aa, 114 kDa
Observed Molecular Weight114 kDa
GenBank Accession NumberBC032370
Gene Symbol NNT
Gene ID (NCBI) 23530
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

Protocols

Product Specific Protocols
WB protocol for NNT antibody 68194-1-IgDownload protocol
IHC protocol for NNT antibody 68194-1-IgDownload protocol
IF protocol for NNT antibody 68194-1-IgDownload protocol
IP protocol for NNT antibody 68194-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols