LNCaP cells were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
LNCaP cells were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 68194-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 68194-1-Ig
Various lysates (mouse heart tissue and mouse liver tissue were dissected from BALB/c mice) were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates (mouse heart tissue and mouse liver tissue were dissected from BALB/c mice) were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 68194-1-Ig
IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug.
IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug.
IHC staining of human liver using 68194-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 68194-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 68194-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 68194-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 68194-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
pig heart tissue, LNCaP cells, HeLa cells, Jurkat cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat liver tissue, mouse liver tissue
Positive IP detected in
HepG2 cells
Positive IHC detected in
human liver tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68194-1-Ig targets NNT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).
LNCaP cells were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 68194-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 68194-1-Ig
Various lysates (mouse heart tissue and mouse liver tissue were dissected from BALB/c mice) were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver using 68194-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 68194-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 68194-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 68194-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HepG2 using 68194-1-Ig
IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug.
IF/ICC Figures
IF Staining of HeLa using 68194-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 68194-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
NNT Monoclonal antibody
Catalog Number
68194-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:400-1:1600
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat, rabbit, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
