NMI Recombinant antibody, PBS Only (Capture)

NMI Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241135A9

Cat no : 83986-2-PBS

Synonyms

N-myc-interactor, N-myc and STAT interactor, N myc interactor, N myc and STAT interactor, N myc (and STAT) interactor

---

Validation Data Gallery

• 

  WB analysis using 83986-2-RR (same clone as 83986-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83986-2-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83986-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83986-2-RR (same clone as 83986-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-2-RR, Clone:241135A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83986-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00891-1

  Cytometric bead array standard curve of MP00891-1, NMI Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83986-2-PBS. Detection antibody: 83986-4-PBS. Standard: Ag34759. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00891-3

  Cytometric bead array standard curve of MP00891-3, NMI Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83986-2-PBS. Detection antibody: 83986-3-PBS. Standard: Ag34759. Range: 0.625-80 ng/mL.

---

Product Information

83986-2-PBS targets NMI as part of a matched antibody pair:

MP00891-1: 83986-2-PBS capture and 83986-4-PBS detection (validated in Cytometric bead array)

MP00891-3: 83986-2-PBS capture and 83986-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NMI fusion protein Ag34759
• Full Name: N-myc (and STAT) interactor
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001268
• Gene Symbol: NMI
• Gene ID (NCBI): 9111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.