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NMI Recombinant antibody

NMI Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241135A9

Cat no : 83986-2-RR

Synonyms

N-myc-interactor, N-myc and STAT interactor, N myc interactor, N myc and STAT interactor, N myc (and STAT) interactor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NMI Recombinant antibody (83986-2-RR)

    WB analysis using 83986-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83986-2-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using NMI Recombinant antibody (83986-2-RR)

    FC experiment of HepG2 using 83986-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-2-RR, Clone:241135A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, PC-3 cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83986-2-RR is NMI Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NMI fusion protein Ag34759
Full Name N-myc (and STAT) interactor
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC001268
Gene Symbol NMI
Gene ID (NCBI) 9111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.

Protocols

Product Specific Protocols
WB protocol for NMI antibody 83986-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols