NLRP6 Recombinant antibody

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  WB analysis of mouse colon using 83555-5-RR

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 83555-5-RR (NLRP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 83555-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NLRP6 antibody (83555-5-RR, Clone: 240543B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83555-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83555-5-RR against Human NLRP6 were performed. The affinity constant is 3.65 nM.

NLRP6 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83555-5-RR

Clone No.240543B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

PYPAF5, NALP6, 240543B2

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83555-5-RR targets NLRP6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NLRP6 fusion protein Ag34516
• Full Name: NLR family, pyrin domain containing 6
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: NM_138329.2
• Gene Symbol: NLRP6
• Gene ID (NCBI): 171389
• RRID: AB_3671172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P59044
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOD-like receptor family pyrin domain containing 6 (NLRP6) is also named as NACHT, LRR and PYD domains-containing protein 6 and ZFML, Angiotensin II/vasopressin receptor, NALP6 and PYPAF5. NLRP6 forms a complex with ASC and caspase-1 or caspase-11 to form an inflammasome complex cleaving pro-interleukin-1β (IL-1β) and IL-18 into their biologically active forms. The function of NLRP6 has been attributed to the maintenance of epithelial integrity and host defense against microbial infections (PMID: 33910012). NLRP6 is reported to involved in inflammasome activation, regulation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, antiviral interferon (IFN) signaling, mucus secretion, and antimicrobial peptide (AMP) production. NLRP6 is a novel NLR family member, that shows high expression in the intestine and liver (in contrast to NLRP3 in myeloid cells), to regulate inflammation and host defense against microbes (PMID: 32386845).