Tested Applications
Positive WB detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83123-6-RR targets NLRP12 in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | NLRP12 fusion protein Ag34281 Predict reactive species |
Full Name | NLR family, pyrin domain containing 12 |
Calculated Molecular Weight | 1061 aa, 120 kDa |
Observed Molecular Weight | 95-110 kDa |
GenBank Accession Number | BC028069 |
Gene Symbol | NLRP12 |
Gene ID (NCBI) | 91662 |
RRID | AB_3670832 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P59046 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).
Protocols
Product Specific Protocols | |
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WB protocol for NLRP12 antibody 83123-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |