NLRP12 Recombinant antibody, PBS Only

• 

  WB analysis using 83123-6-RR (same clone as 83123-6-PBS)

  Mouse liver tissue was subjected to SDS PAGE followed by western blot with 83123-6-RR (NLRP12 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83123-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83123-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83123-6-RR against Human NLRP12 were performed. The affinity constant is 0.236 nM.

NLRP12 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 83123-6-PBS

Clone No.241838E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1, Monarch 1

---

Product Information

83123-6-PBS targets NLRP12 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NLRP12 fusion protein Ag34281
• Full Name: NLR family, pyrin domain containing 12
• Calculated Molecular Weight: 1061 aa, 120 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: BC028069
• Gene Symbol: NLRP12
• Gene ID (NCBI): 91662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P59046
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).