NLRP10 Recombinant antibody

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  WB analysis of mouse heart using 83131-8-RR

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 83131-8-RR (NLRP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 83131-8-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of HeLa using 83131-8-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83131-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83131-8-RR against Human NLRP10 were performed. The affinity constant is 0.899 nM.

NLRP10 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83131-8-RR

Clone No.230402C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Nucleotide-binding oligomerization domain protein 8, NOD8, NALP10, NACHT, LRR and PYD domains-containing protein 10, CLR11.1

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive FC (Intra) detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83131-8-RR targets NLRP10 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NLRP10 fusion protein Ag26402
• Full Name: NLR family, pyrin domain containing 10
• Calculated Molecular Weight: 655 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC104957
• Gene Symbol: NLRP10
• Gene ID (NCBI): 338322
• RRID: AB_3670834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q86W26
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NLRP10 is a member of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). NLRP10 likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.