NLRP10 Recombinant antibody, PBS Only

NLRP10 Uni-rAb^TM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230402C1

Cat no : 83131-8-PBS

Synonyms

Nucleotide-binding oligomerization domain protein 8, NOD8, NALP10, NACHT, LRR and PYD domains-containing protein 10, CLR11.1

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Validation Data Gallery

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  WB analysis of mouse heart using 83131-8-RR (same clone as 83131-8-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 83131-8-RR (NLRP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83131-8-RR (same clone as 83131-8-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83131-8-RR (same clone as 83131-8-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83131-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83131-8-RR against Human NLRP10 were performed. The affinity constant is 0.899 nM.

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Product Information

The immunogen of 83131-8-PBS is NLRP10 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NLRP10 fusion protein Ag26402
• Full Name: NLR family, pyrin domain containing 10
• Calculated Molecular Weight: 655 aa, 75 kDa
• GenBank Accession Number: BC104957
• Gene Symbol: NLRP10
• Gene ID (NCBI): 338322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.