CoraLite®594-conjugated NINJ2 Monoclonal antibody

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  FC experiment of U2OS using CL594-66202

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite®594 Ninj2 Monoclonal Antibody (CL594-66202, Clone:5C5B2)(red), or 0.4 ug CoraLite®594 Mouse IgG2b isotype control (11B8C4) (CL594-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

NINJ2 Monoclonal Antibody for FC (Intra)

Cat No. CL594-66202

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

Ninjurin-2, Nerve injury-induced protein 2, 5C5B2

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Tested Applications

• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66202 targets NINJ2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NINJ2 fusion protein Ag5343
• Full Name: ninjurin 2
• Calculated Molecular Weight: 142 aa, 16 kDa
• GenBank Accession Number: BC057766
• Gene Symbol: NINJ2
• Gene ID (NCBI): 4815
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZG7
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596)