NGAL Recombinant antibody, PBS Only (Detector)

NGAL Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

2L8

Cat no : 82841-2-PBS

Synonyms

LCN2, lipocalin 2, Lcn 2, HNL, 2L8

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00770-1

  Sandwich ELISA standard curve of MP00770-1, NGAL Recombinant Matched Antibody Pair - PBS only. 82841-9-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0268. 82841-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

• 

  Sample test of MP00770-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 days. The mean NGAL concentration was determined to be 18,080.8 pg/mL in unstimulated PBMC supernatant, 32,268.8 pg/mL in PHA stimulated PBMC supernatant.

• 

  Sample test of MP00770-1

  Saliva of eight individual healthy human donors was measured. The NGAL concentration of detected samples was determined to be 158,732.5 pg/mL with a range of 52,136.9 - 388,400.2 pg/mL.

• 

  Sample test of MP00770-1

  Urine of eight individual healthy human donors was measured. The NGAL concentration of detected samples was determined to be 11,654.6 pg/mL with a range of 1,964.2 - 21,150.4 pg/mL.

• 

  Sample test of MP00770-1

  Plasma of fifteen individual healthy human donors was measured. The NGAL concentration of detected samples was determined to be 38,260.8 pg/mL with a range of 17,778.7 - 90,593.8 pg/mL.

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Product Information

82841-2-PBS targets NGAL as part of a matched antibody pair:

MP00770-1: 82841-9-PBS capture and 82841-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lipocalin 2
• Calculated Molecular Weight: 23kd
• GenBank Accession Number: NP_005555.2
• Gene Symbol: NGAL/LCN2
• Gene ID (NCBI): 3934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.