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NGAL Recombinant antibody, PBS Only (Capture)

NGAL Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

2N10

Cat no : 82841-9-PBS

Synonyms

LCN2, lipocalin 2, Lcn 2, HNL, 2N10

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00770-1

    Sandwich ELISA standard curve of MP00770-1

    Sandwich ELISA standard curve of MP00770-1, NGAL Recombinant Matched Antibody Pair - PBS only. 82841-9-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0268. 82841-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

  • Sample test of MP00770-1

    Sample test of MP00770-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 days. The mean NGAL concentration was determined to be 18,080.8 pg/mL in unstimulated PBMC supernatant, 32,268.8 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00770-1

    Sample test of MP00770-1

    Saliva of eight individual healthy human donors was measured. The NGAL concentration of detected samples was determined to be 158,732.5 pg/mL with a range of 52,136.9 - 388,400.2 pg/mL.

  • Sample test of MP00770-1

    Sample test of MP00770-1

    Urine of eight individual healthy human donors was measured. The NGAL concentration of detected samples was determined to be 11,654.6 pg/mL with a range of 1,964.2 - 21,150.4 pg/mL.

  • Sample test of MP00770-1

    Sample test of MP00770-1

    Plasma of fifteen individual healthy human donors was measured. The NGAL concentration of detected samples was determined to be 38,260.8 pg/mL with a range of 17,778.7 - 90,593.8 pg/mL.

Product Information

82841-9-PBS targets NGAL as part of a matched antibody pair:

MP00770-1: 82841-9-PBS capture and 82841-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name lipocalin 2
Calculated Molecular Weight23kd
GenBank Accession NumberNP_005555.2
Gene Symbol NGAL/LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.