NGAL Recombinant antibody

• Featured Product
• KD/KO Validated

NGAL Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G1

Cat no : 82841-1-RR

Synonyms

LCN2, lipocalin 2, Lcn 2, HNL, 2G1

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Validation Data Gallery

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  WB analysis of HT-29 using 82841-1-RR

  WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells.

• 

  WB analysis using 82841-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82841-1-RR (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82841-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82841-1-RR against Human NGAL were performed. The affinity constant is 0.776 nM.

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Tested Applications

• Positive WB detected in: human saliva, HT-29 cells, SW480 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82841-1-RR targets NGAL in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lipocalin 2
• Calculated Molecular Weight: 23kd
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: NP_005555.2
• Gene Symbol: NGAL/LCN2
• Gene ID (NCBI): 3934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).