NGAL Recombinant antibody, PBS Only

NGAL Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G1

Cat no : 82841-1-PBS

Synonyms

24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25

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Validation Data Gallery

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  WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

  WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

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  WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82841-1-PBS targets NGAL in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lipocalin 2
• Calculated Molecular Weight: 23kd
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: NP_005555.2
• Gene Symbol: LCN2
• Gene ID (NCBI): 3934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).