NFKB1,p105,p50 Monoclonal antibody, PBS Only

NFKB1,p105,p50 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G1E3

Cat no : 66992-1-PBS

Synonyms

DKFZp686C01211, DNA binding factor KBF1, EBP 1, KBF1, NF kappa B, NFKB p105, NFKB p50, NFKB1, NFKB1,p105, NFKB1,p105,p50, p105, p50

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Validation Data Gallery

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  WB analysis using 66992-1-Ig (same clone as 66992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFKB1,p105,p50 antibody (66992-1-Ig, Clone: 2G1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66992-1-Ig (same clone as 66992-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (66992-1-Ig, Clone:2G1E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66992-1-PBS targets NFKB1,p105,p50 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NFKB1,p105,p50 fusion protein Ag5832
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 50 kDa, 105 kDa
• GenBank Accession Number: BC051765
• Gene Symbol: NFKB1
• Gene ID (NCBI): 4790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.