NFIC Monoclonal antibody, PBS Only

NFIC Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11A5

Cat no : 68318-1-PBS

Synonyms

NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor

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Validation Data Gallery

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  WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68318-1-Ig (same clone as 68318-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFIC Monoclonal antibody (68318-1-Ig, Clone:1A11A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

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Product Information

68318-1-PBS targets NFIC in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NFIC fusion protein Ag9472
• Full Name: nuclear factor I/C (CCAAT-binding transcription factor)
• Calculated Molecular Weight: 428aa,48 kDa; 508aa,57 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC012120
• Gene Symbol: NFIC
• Gene ID (NCBI): 4782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.