NF90/ILF3 Monoclonal antibody, PBS Only (Capture)

NF90/ILF3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H10G3

Cat no : 68213-1-PBS

Synonyms

ILF3, IL-F3, DRBP76, DRBF, CBTF

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Validation Data Gallery

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  WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 68213-1-Ig (same clone as 68213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50472-3

  Cytometric bead array standard curve of MP50472-3, NF90,ILF3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68213-1-PBS. Detection antibody: 68213-4-PBS. Standard:Ag30868. Range: 0.781-100 ng/mL.

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Product Information

68213-1-PBS targets NF90/ILF3 as part of a matched antibody pair:

MP50472-3: 68213-1-PBS capture and 68213-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NF90/ILF3 fusion protein Ag30868
• Full Name: interleukin enhancer binding factor 3, 90kDa
• Calculated Molecular Weight: 894 aa, 95 kDa
• Observed Molecular Weight: 90 kDa, 110 kDa
• GenBank Accession Number: BC064836
• Gene Symbol: ILF3
• Gene ID (NCBI): 3609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.