NEUROD2 Monoclonal antibody

NEUROD2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C7B3

Cat no : 68284-1-Ig

Synonyms

bHLHa1, NDRF, NeuroD related factor, NEUROD2, neurogenic differentiation 2

---

Validation Data Gallery

• 

  WB analysis using 68284-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68284-1-Ig (NEUROD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of mouse cerebellum using 68284-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68284-1-Ig (NEUROD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: pig brain tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68284-1-Ig targets NEUROD2 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NEUROD2 fusion protein Ag26068
• Full Name: neurogenic differentiation 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41-50 kDa
• GenBank Accession Number: BC022481
• Gene Symbol: NEUROD2
• Gene ID (NCBI): 4761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEUROD2 (Neurogenic differentiation factor 2), is transcriptional regulator that regulates early neuronal differentiation during development (PMID: 7754368). NeuroD2 is expressed in the postnatal cortex and mediate the activity-dependent refinement of thalamocortical axon terminals (PMID: 17453014). NeuroD2 is mainly involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Mutantions in NEUROD2 can cause neurodevelopmental disorders including intellectual disability and autism spectrum disorders (PMID: 34188164).