NELF Recombinant antibody, PBS Only (Detector)

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  WB analysis using 83490-2-RR (same clone as 83490-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83490-2-RR (NELF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83490-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00490-1

  Sandwich ELISA standard curve of MP00490-1, Human NELF Recombinant Matched Antibody Pair - PBS only. 83490-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34902. 83490-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

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  Sample test of MP00490-1

  The mean NELF concentration was determined to be 45.6 ng/mL in HepG2 cell extract based on a 2.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP00490-1

  Cytometric bead array standard curve of MP00490-1, NELF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83490-1-PBS. Detection antibody: 83490-2-PBS. Standard: Ag34902. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00490-2

  Cytometric bead array standard curve of MP00490-2, NELF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83490-3-PBS. Detection antibody: 83490-2-PBS. Standard: Ag34902. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83490-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83490-2-RR against Human NELF were performed. The affinity constant is below 1 pM.

NELF Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83490-2-PBS

Clone No.240482G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor, 240482G1

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Product Information

83490-2-PBS targets NELF as part of a matched antibody pair:

MP00490-1: 83490-1-PBS capture and 83490-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00490-2: 83490-3-PBS capture and 83490-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NELF fusion protein Ag34902
• Full Name: nasal embryonic LHRH factor
• Calculated Molecular Weight: 507 aa, 56 kDa
• GenBank Accession Number: BC016862
• Gene Symbol: NELF
• Gene ID (NCBI): 26012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6X4W1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.