NEDD4 Recombinant antibody, PBS Only (Capture/Detector)

NEDD4 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230478A1

Cat no : 83112-2-PBS

Synonyms

KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00234-2

  Sandwich ELISA standard curve of MP00234-2, Human NEDD4 Recombinant Matched Antibody Pair - PBS only. 83112-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16291. 83112-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00234-2

  The mean NEDD4 concentration was determined to be 14.63 ng/mL in C6 cell extract based on a 2.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP00234-1

  Cytometric bead array standard curve of MP00234-1, NEDD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83112-2-PBS. Detection antibody: 83112-4-PBS. Standard: Ag16291. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 83112-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83112-2-PBS against Human NEDD4 was performed. The affinity constant is 4.26 nM.

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Product Information

83112-2-PBS targets NEDD4 as part of a matched antibody pair:

MP00234-1: 83112-2-PBS capture and 83112-4-PBS detection (validated in Cytometric bead array)

MP00234-2: 83112-5-PBS capture and 83112-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEDD4 fusion protein Ag16291
• Full Name: neural precursor cell expressed, developmentally down-regulated 4
• Calculated Molecular Weight: 1319 aa, 149 kDa
• GenBank Accession Number: BC152452
• Gene Symbol: NEDD4
• Gene ID (NCBI): 4734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.