NDUFV2 Monoclonal antibody, PBS Only

NDUFV2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig, Chicken

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B4A5

Cat no : 68150-1-PBS

Synonyms

NDUFV2

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Validation Data Gallery

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  WB analysis using 68150-1-Ig (same clone as 68150-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

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  WB analysis of chicken heart using 68150-1-Ig (same clone as 68150-1-PBS)

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68150-1-Ig (same clone as 68150-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

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Product Information

68150-1-PBS targets NDUFV2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFV2 fusion protein Ag7760
• Full Name: NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 24-27 kDa
• GenBank Accession Number: BC001632
• Gene Symbol: NDUFV2
• Gene ID (NCBI): 4729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH: ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).