NDUFV2 Monoclonal antibody

NDUFV2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig, Chicken and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3B4A5

Cat no : 68150-1-Ig

Synonyms

NDUFV2

---

Validation Data Gallery

• 

  WB analysis using 68150-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of chicken heart using 68150-1-Ig

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68150-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 68150-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 68150-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells, chicken heart tissue, HeLa cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68150-1-Ig targets NDUFV2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig, Chicken samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFV2 fusion protein Ag7760
• Full Name: NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 24-27 kDa
• GenBank Accession Number: BC001632
• Gene Symbol: NDUFV2
• Gene ID (NCBI): 4729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH: ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).

Publications

Species	Application	Title
rat	WB	Exp Mol Pathol; Proteomic analysis of the effects of Dictyophora polysaccharide on arsenic-induced hepatotoxicity in rats; Authors - Xi Yan