Filter: AllWBIHC

WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.
WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.
WB analysis of 4T1 using 68144-1-Ig (same clone as 68144-1-PBS)
4T1 cells were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.
WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 68144-1-Ig (same clone as 68144-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 68144-1-Ig (same clone as 68144-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 68144-1-Ig (same clone as 68144-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 68144-1-Ig (same clone as 68144-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

NDUFV1 Monoclonal antibody, PBS Only

NDUFV1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 68144-1-PBS

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rabbit, rat, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68144-1-PBS targets NDUFV1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.

Tested Reactivity	Human, pig, rabbit, rat, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	NDUFV1 fusion protein Ag32659 Predict reactive species
Full Name	NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	45 kDa
GenBank Accession Number	BC015645
Gene Symbol	NDUFV1
Gene ID (NCBI)	4723
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P49821
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).