NDUFV1 Monoclonal antibody

NDUFV1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rabbit, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E9D4

Cat no : 68144-1-Ig

Synonyms

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

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Validation Data Gallery

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  WB analysis using 68144-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68144-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68144-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 68144-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 68144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 68144-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 68144-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-12 cells, A549 cells, 4T1 cells, pig heart tissue, HeLa cells, HEK-293 cells, H9C2 cells, rabbit heart tissue
• Positive IHC detected in: human lung cancer tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68144-1-Ig targets NDUFV1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.

• Tested Reactivity: Human, pig, rabbit, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFV1 fusion protein Ag32659
• Full Name: NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC015645
• Gene Symbol: NDUFV1
• Gene ID (NCBI): 4723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).