NDUFB8 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NDUFB8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14794-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14794-1-AP (same clone as 14794-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  WB analysis of various samples using 14794-1-AP (same clone as 14794-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 14794-1-AP (same clone as 14794-1-PBS)

  WB result of NDUFB8 antibody (14794-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14794-1-AP (same clone as 14794-1-PBS)

  IP result of anti-NDUFB8 (IP:14794-1-AP, 4ug; Detection:14794-1-AP 1:2000) with HepG2 cells lysate 880 ug. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14794-1-AP (same clone as 14794-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (14794-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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Product Information

14794-1-PBS targets NDUFB8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFB8 fusion protein Ag6495
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC000466
• Gene Symbol: NDUFB8
• Gene ID (NCBI): 4714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.