NDUFA4L2 Monoclonal antibody, PBS Only (Capture)

NDUFA4L2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1H10

Cat no : 66050-1-PBS

Synonyms

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10

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Validation Data Gallery

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  WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IF Staining of OS-RC-2 using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:1200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunofluorescent analysis of Hela cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66050-1-Ig (same clone as 66050-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66050-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50529-1

  Cytometric bead array standard curve of MP50529-1, NDUFA4L2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66050-1-PBS. Detection antibody: 66050-2-PBS. Standard:Ag9233. Range: 0.098-100 ng/mL.

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Product Information

66050-1-PBS targets NDUFA4L2 as part of a matched antibody pair:

MP50529-1: 66050-1-PBS capture and 66050-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFA4L2 fusion protein Ag9233
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
• Calculated Molecular Weight: 87 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC011910
• Gene Symbol: NDUFA4L2
• Gene ID (NCBI): 56901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.