NDE1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NDE1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10233-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10233-1-AP (same clone as 10233-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  WB analysis of A2780 using 10233-1-AP (same clone as 10233-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10233-1-AP (same clone as 10233-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10233-1-AP (same clone as 10233-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 10233-1-AP (same clone as 10233-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 10233-1-AP (same clone as 10233-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10233-1-AP (same clone as 10233-1-PBS)

  IP result of anti-NDE1 (IP:10233-1-AP, 3ug; Detection:10233-1-AP 1:1500) with HEK-293 cells lysate 4500ug. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10233-1-AP (same clone as 10233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10233-1-AP (same clone as 10233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 10233-1-AP (same clone as 10233-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

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Product Information

10233-1-PBS targets NDE1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDE1 fusion protein Ag0336
• Full Name: nudE nuclear distribution gene E homolog 1 (A. nidulans)
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC001421
• Gene Symbol: NDE1
• Gene ID (NCBI): 54820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.