NCOA4 Recombinant antibody, PBS Only (Capture)

NCOA4 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240169H7

Cat no : 83394-4-PBS

Synonyms

ELE1, ARA70, Androgen receptor-associated protein of 70 kDa, 70 kDa AR-activator, 70 kDa AR activator

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Validation Data Gallery

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  WB analysis using 83394-4-RR (same clone as 83394-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83394-4-RR (NCOA4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83394-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83394-4-RR (same clone as 83394-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NCOA4 antibody (83394-4-RR, Clone: 240169H7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83394-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83394-4-RR (same clone as 83394-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NCOA4 Recombinant antibody (83394-4-RR, Clone:240169H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83394-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00435-1

  Cytometric bead array standard curve of MP00435-1, NCOA4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83394-4-PBS. Detection antibody: 83394-2-PBS. Standard: Ag15277. Range: 3.13-100 ng/mL.

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  Affinity and Kinetic Characterization of 83394-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83394-4-PBS against Human NCOA4 was performed. The affinity constant is 0.119 nM.

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  Affinity and Kinetic Characterization of 83394-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83394-4-RR against Human NCOA4 were performed. The affinity constant is 0.586 nM.

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Product Information

83394-4-PBS targets NCOA4 as part of a matched antibody pair:

MP00435-1: 83394-4-PBS capture and 83394-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NCOA4 fusion protein Ag15277
• Full Name: nuclear receptor coactivator 4
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70-73 kDa
• GenBank Accession Number: BC012736
• Gene Symbol: NCOA4
• Gene ID (NCBI): 8031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nuclear receptor coactivator 4 (NCOA4) also named androgen receptor (AR) coactivator ARA70, RFG and ELE1, is a putative co-activator that specifically enhances the activity of the androgen receptor. In human thyroid carcinomas, the Ret proto-oncogene fuses to ARA70 to form Ret/PTC3 by an intrachromosomal inversion of chromosome 10 in vivo. ARA70a can function as a ligand-enhanced co-activator of PPARg in adipocytes. However, PPARg-ARA70 transactivation can be squelched by AR, which suggests cross talk between PPARg- and AR-mediated response. ARA70a has no intrinsic transcription activation domain or histone acetyltransferase activity, but it interacts with histone acetyltransferase, p/CAF, CBP and p300/CBP-associated factors, and the basal transcription factor TFIIB. The interaction between ARA70 and AR occurs through the ligand-binding domain. The presence of ARA70 can enhance the androgenic activity of 17-b Estradiol (E2) and antiandrogens toward AR. ARA70 may be involved in prostate carcinogenesis and ovarian cancer and may serve as a key mediator of estrogen-androgen synergism.