NCAPG Monoclonal antibody, PBS Only

NCAPG Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4D3

Cat no : 68082-1-PBS

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

---

Validation Data Gallery

• 

  WB analysis using 68082-1-Ig (same clone as 68082-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68082-1-Ig (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 68082-1-Ig (same clone as 68082-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCAPG antibody (68082-1-Ig, Clone: 2D4D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 68082-1-Ig (same clone as 68082-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human NCAPG (68082-1-Ig, Clone:2D4D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68082-1-PBS targets NCAPG in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCAPG fusion protein Ag19926
• Full Name: non-SMC condensin I complex, subunit G
• Calculated Molecular Weight: 1015 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC068467
• Gene Symbol: NCAPG
• Gene ID (NCBI): 64151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.