NCAPG Monoclonal antibody

NCAPG Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D4D3

Cat no : 68082-1-Ig

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

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Validation Data Gallery

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  WB analysis using 68082-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68082-1-Ig (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  IF Staining of U2OS using 68082-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCAPG antibody (68082-1-Ig, Clone: 2D4D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of U2OS using 68082-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human NCAPG (68082-1-Ig, Clone:2D4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells
• Positive FC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68082-1-Ig targets NCAPG in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCAPG fusion protein Ag19926
• Full Name: non-SMC condensin I complex, subunit G
• Calculated Molecular Weight: 1015 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC068467
• Gene Symbol: NCAPG
• Gene ID (NCBI): 64151
• RRID: AB_2918819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.