PE Anti-Human NCAM1/CD56 Rabbit Recombinant Antibody

NCAM1/CD56 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

240078A7

Cat no : PE-98033

Synonyms

CD56, NCAM1, 240078A7, NCAM 1, NCAM

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Validation Data Gallery

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  FC experiment of human peripheral blood lymphocytes using PE-98033

  1x10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 and 5 ul PE Anti-Human NCAM1/CD56 Rabbit Recombinant Antibody (PE-98033, Clone: 240078A7) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using PE-98033

  1x10^6 human peripheral blood lymphocytes were surface stained with 5 ul PE Anti-Human NCAM1/CD56 Rabbit Recombinant Antibody (PE-98033, Clone: 240078A7) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of PE-98033 is NCAM1/CD56 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: neural cell adhesion molecule 1
• Calculated Molecular Weight: 95 kDa
• GenBank Accession Number: BC047244
• Gene Symbol: NCAM1
• Gene ID (NCBI): 4684
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.