NCAM1/CD56 Monoclonal antibody, PBS Only

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  WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 60238-1-Ig (same clone as 60238-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 60238-1-Ig (same clone as 60238-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 60238-1-Ig (same clone as 60238-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

NCAM1/CD56 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 60238-1-PBS

Clone No.1E8C9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CD56, NCAM1, 1E8C9, NCAM 1, NCAM

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Product Information

60238-1-PBS targets NCAM1/CD56 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCAM1/CD56 fusion protein Ag5732
• Full Name: neural cell adhesion molecule 1
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC047244
• Gene Symbol: NCAM1
• Gene ID (NCBI): 4684
• ENSEMBL Gene ID: ENSG00000149294
• RRID: AB_2881361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13591
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).