NCALD Monoclonal antibody, PBS Only

NCALD Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B4E10

Cat no : 66088-1-PBS

Synonyms

NCALD, neurocalcin delta, neurocalcin δ

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Validation Data Gallery

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  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66088-1-Ig (same clone as 66088-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66088-1-PBS targets NCALD in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCALD fusion protein Ag18760
• Full Name: neurocalcin delta
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC036098
• Gene Symbol: NCALD
• Gene ID (NCBI): 83988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.