NBR1 Monoclonal antibody, PBS Only

NBR1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B2

Cat no : 68062-1-PBS

Synonyms

1A1 3B, 1A13B, FLJ55359, FLJ98272, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B

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Validation Data Gallery

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  WB analysis using 68062-1-Ig (same clone as 68062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68062-1-Ig (NBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68062-1-PBS in a different storage buffer formulation.

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Product Information

68062-1-PBS targets NBR1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NBR1 fusion protein Ag8652
• Full Name: neighbor of BRCA1 gene 1
• Calculated Molecular Weight: 966 aa, 107 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC009808
• Gene Symbol: NBR1
• Gene ID (NCBI): 4077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).