NBR1 Monoclonal antibody

NBR1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F2B2

Cat no : 68062-1-Ig

Synonyms

1A1 3B, 1A13B, FLJ55359, FLJ98272, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B

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Validation Data Gallery

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  WB analysis using 68062-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68062-1-Ig (NBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NCCIT cells, hTERT-RPE1 cells, HeLa cells, HEK-293 cells, K-562 cells, rat testis tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68062-1-Ig targets NBR1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NBR1 fusion protein Ag8652
• Full Name: neighbor of BRCA1 gene 1
• Calculated Molecular Weight: 966 aa, 107 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC009808
• Gene Symbol: NBR1
• Gene ID (NCBI): 4077
• RRID: AB_2918803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).