NBCe2 Polyclonal antibody

NBCe2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26150-1-AP

Synonyms

NBC4, NBCe2, SLC4A5

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Validation Data Gallery

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  IHC staining of human liver cancer using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26150-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26150-1-AP (NBCe2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26150-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NBCe2 antibody (26150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 26150-1-AP is NBCe2 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NBCe2 fusion protein Ag23558
• Full Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
• Observed Molecular Weight: 108-127 kDa
• GenBank Accession Number: BC109221
• Gene Symbol: SLC4A5
• Gene ID (NCBI): 57835
• RRID: AB_2918099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBCe2, also named as SLC4A5 and NBC4, belongs to the anion exchanger (TC 2.A.31) family. NBCe2 mediates electrogenic Na+:HCO3 − transport, and can operate in both a 1:2, and 1:3 manner, but the transport stoichiometry as well as directionality have not been defined for the renal NBCe2. The expression of NBCe2 has been reported in nearly all kidney nephron segments and CDs, indicating that some species-specificity might be present (PMID: 32547422). NBCe2 has 8 isoforms with the molecular mass of 108-127 kDa.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Cell-Type Dependent Regulation of the Electrogenic Na+/HCO3- Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma; Authors - Marina Giannaki
mouse	WB	Neuron; The sodium-bicarbonate cotransporter Slc4a5 mediates feedback at the first synapse of vision; Authors - Rei Morikawa