NAMPT/PBEF Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NAMPT/PBEF Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D4D8

Cat no : 66385-1-PBS

Synonyms

1110035O14Rik, NAmPRTase, NAMPT, NAMPT/PBEF, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

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Validation Data Gallery

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  WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66385-1-Ig (same clone as 66385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 66385-1-Ig (same clone as 66385-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 66385-1-Ig (same clone as 66385-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66385-1-Ig (same clone as 66385-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 66385-1-Ig (same clone as 66385-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66385-1-Ig (same clone as 66385-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66385-1-Ig (NAMPT/PBEF antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66385-1-Ig (same clone as 66385-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NAMPT/PBEF antibody (66385-1-Ig, Clone: 3D4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66385-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66385-1-PBS targets NAMPT/PBEF in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NAMPT/PBEF fusion protein Ag2434
• Full Name: nicotinamide phosphoribosyltransferase
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC020691
• Gene Symbol: NAMPT
• Gene ID (NCBI): 10135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.