CoraLite® Plus 647-conjugated N-cadherin Monoclonal antibody

• Featured Product
• KD/KO Validated

N-cadherin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat, rabbit

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D8B3

Cat no : CL647-66219

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

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Validation Data Gallery

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  IF Staining of mouse heart using CL647-66219

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 N-cadherin antibody (CL647-66219, Clone: 1D8B3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66219 targets N-cadherin in IF applications and shows reactivity with human, pig, mouse, rat, rabbit samples.

• Tested Reactivity: human, pig, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: N-cadherin fusion protein Ag4996
• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC036470
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).