N-cadherin Monoclonal antibody, PBS Only

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G7C2

Cat no : 68532-1-PBS

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

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Validation Data Gallery

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  WB analysis using 68532-1-Ig (same clone as 68532-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

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  WB analysis of hTERT-RPE1 using 68532-1-Ig (same clone as 68532-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 68532-1-Ig (same clone as 68532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68532-1-PBS in a different storage buffer formulation.

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Product Information

68532-1-PBS targets N-cadherin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Pig, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001792.4
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).