N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2G7C2

Cat no : 68532-1-Ig

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin



Tested Applications

Positive WB detected inpig heart tissue, hTERT-RPE1 cells, rabbit heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68532-1-Ig targets N-cadherin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Pig, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated Molecular Weight100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

Protocols

Product Specific Protocols
WB protocol for N-cadherin antibody 68532-1-IgDownload protocol
IHC protocol for N-cadherin antibody 68532-1-IgDownload protocol
IF protocol for N-cadherin antibody 68532-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols