Various lysates were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of hTERT-RPE1 using 68532-1-Ig
hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse heart using 68532-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 68532-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse heart using 68532-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
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mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse heart tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68532-1-Ig targets N-cadherin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).
Various lysates were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of hTERT-RPE1 using 68532-1-Ig
hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68532-1-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse heart using 68532-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 68532-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68532-1-Ig (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse heart using 68532-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of mouse heart using 68532-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (68532-1-Ig, Clone: 2G7C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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