MultiProTM 5CFLX Anti-Human IL-6 (4G3E6)

NeutraKine® IL-6 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

CloneNo.

4G3E6

Cat no : G69001-1-5C

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6, NeutraKine® IL-6

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Validation Data Gallery

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  Single Cell Sequencing experiment G69001-1-5C on Resting PBMC

  G69001-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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  Single Cell Sequencing experiment G69001-1-5C on PBMC treated with 4hr LPS + TI

  G69001-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

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Tested Applications

• Positive Single Cell (Intra) detected in: 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Recommended dilution

• SINGLE CELL (INTRA): <0.5ug/test

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G69001-1-5C targets NeutraKine® IL-6 in Single Cell (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Oligo Conjugate
• Type: Monoclonal
• Immunogen: NeutraKine® IL-6 fusion protein HZ-1019
• Full Name: MultiPro^TM 5CFLX Anti-Human IL-6 (4G3E6)
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• Conjugate: 5CFLX
• Form: Liquid
• Storage Buffer: PBS with 1mM EDTA and 0.09% sodium azide
• Storage Conditions: 2-8°C

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

This antibody can be used to neutralize the bioactivity of IL-6.