HumanKine® recombinant human IL-6 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.03-0.24 ng/mL in 3G12B10 cells.

Specific Activity

minimally 3.00 x 10⁷ IU/mg

Species Reactivity

human,mouse,monkey,rabbit,rat

Purity

>95%

Publications

45

Reviews (1)

Cat no : HZ-1019

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

---

Validation Data Gallery

• 

  Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

• 

  Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 range is 0.03-0.24 ng/mL​.

• 

  Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 7TD1 hybridoma cell line. Cell number was quantitatively assessed by CellTiter® Cell Viability Reagent. 7TD1 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 is <0.5 ng/mL​.

---

Technical Specifications

• GeneID: 3569
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.03-0.24 ng/mL in 3G12B10 cells.
• Activity(Secondary): Typically <0.5 ng/mL EC50 in 7TD1 cells.
• Specific Activity: minimally 3.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P05231
• Molecular Mass: 24 and 29 kDa reduced, 21 and 28 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,monkey,rabbit,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Background

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages. IL-6 plays an essential role in the final differentiation of B-cells into immunoglobulin-secreting cells. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity (PMID: 29736207; 29731693).

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

Publications

Species	Title
mouse	Small Methods; Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy; Authors - Shilin Li
human	J Exp Med; Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.; Authors - AndrewVolk
human	Cell Rep; Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1.; Authors - YusukeEndo
human	Mol Metab; IL-6/JAK2-dependent G6PD phosphorylation promotes nucleotide synthesis and supports tumor growth; Authors - Xuemei Qiu
human	Front Immunol; Dendritic cell phenotype and function in a 3D co-culture model of patient-derived metastatic colorectal cancer organoids; Authors - Beatriz Subtil
human	Cancers (Basel); Silibinin Suppresses Tumor Cell-Intrinsic Resistance to Nintedanib and Enhances Its Clinical Activity in Lung Cancer.; Authors - Joaquim Bosch-Barrera

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Srinivas (Verified Customer) (06-17-2024)

The product is pure high reactive, I have used for check the human mast cell line to check the inflamtory response control when compare with IL-33 treatment in Metabolomics test

• Applications: Cell culture
• Cell Tissue Type: LUVA (Human Mat cell line)