CoraLite® Plus 750 Mouse IgG1 Isotype Control (1F8D3)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1F8D3

Reviews (2)

Cat no : CL750-66360-1

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

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Validation Data Gallery

• 

  FC experiment of human PBMCs using CL750-66360-1

  1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 750 Anti-IgG1 Isotype Control (CL750-66360-1, Clone:1F8D3) (red), CoraLite® Plus 750 Anti-Human CD3 (UCHT1) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66360-1 targets IgG1 Isotype Control in FC applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: IgG1 Isotype Control
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sunil (Verified Customer) (08-03-2023)

useful contol as part of a flow cytometry panel

• Applications: Flow Cytometry
• Primary Antibody Dilution: 5ul used per test

FH

Sareena (Verified Customer) (06-20-2023)

Works excellent with PBMC staining

• Applications: Immunofluorescence
• Cell Tissue Type: Igg1 isotope