1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Mouse IgG1 isotype control (CL488-66360-1, Clone: 1F8D3) (red), or 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone: 1C1G12) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Mouse IgG1 isotype control (CL488-66360-1, Clone: 1F8D3) (red), or 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone: 1C1G12) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of human PBMCs using CL488-66360-1
1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-IgG1 Isotype Control (CL488-66360-1, Clone:1F8D3) (red), CL488 Anti-Human CD35 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-IgG1 Isotype Control (CL488-66360-1, Clone:1F8D3) (red), CL488 Anti-Human CD35 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL488-66360-1 targets IgG1 Isotype Control in FC applications and shows reactivity with Megathura crenulata samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
Product Specific Protocols
FC protocol for CL Plus 488 IgG1 Isotype Control antibody CL488-66360-1
1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-IgG1 Isotype Control (CL488-66360-1, Clone:1F8D3) (red), CL488 Anti-Human CD35 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
FC (INTRA) Figures
FC experiment of A431 using CL488-66360-1
1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Mouse IgG1 isotype control (CL488-66360-1, Clone: 1F8D3) (red), or 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone: 1C1G12) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG1 Isotype Control Monoclonal antibody
Catalog Number
CL488-66360-1
Citations
-
Dilutions
Applications
FC
Reactivity
megathura crenulata
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
