Mouse IgG1 isotype control Monoclonal antibody, PBS Only

Mouse IgG1 isotype control Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

megathura crenulata

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F8D3

Cat no : 66360-1-PBS

Synonyms

Mouse IgG1, Kappa light chain

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Validation Data Gallery

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  WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66360-1-Ig (same clone as 66360-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 66360-1-Ig (same clone as 66360-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig, Clone: 1F8D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD3 (65151-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 66360-1-PBS in a different storage buffer formulation.

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Product Information

66360-1-PBS targets Mouse IgG1 isotype control in WB, ELISA applications and shows reactivity with megathura crenulata samples.

• Tested Reactivity: megathura crenulata
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Mouse IgG1 isotype control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).