CoraLite®594-conjugated Mouse-IgG Polyclonal antibody

Mouse-IgG Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10283

Synonyms

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Validation Data Gallery

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  IF Staining of HeLa using CL594-10283

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66031-1-Ig (alpha tubulin antibody) and CL594-10283 (CL594-Rabbit anti-Mouse IgG as secondary antibody at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10283 targets Mouse-IgG in IF/ICC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Mouse-IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50-55 kDa
• RRID: AB_2919726
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody detectes all the heavy and light chains of total mouse IgG.