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Mouse CD147 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP01320-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01320-4, Mouse CD147 Recombinant Matched Antibody Pair - PBS only. 84488-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1530. 84488-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

    Sandwich ELISA standard curve of MP01320-4

    Sandwich ELISA standard curve of MP01320-4, Mouse CD147 Recombinant Matched Antibody Pair - PBS only. 84488-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1530. 84488-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Serum of six mice was measured. The mouse CD147 concentration of detected samples was determined to be 9,267.4 pg/mL with a range of 6,072.1 - 10,344.1 pg/mL.

    Sample test of MP01320-4

    Serum of six mice was measured. The mouse CD147 concentration of detected samples was determined to be 9,267.4 pg/mL with a range of 6,072.1 - 10,344.1 pg/mL.

  • Urine of six mice was measured. The mouse CD147 concentration of detected samples was determined to be 6,220.5 pg/mL with a range of 2,528.0 - 8372.5 pg/mL.

    Sample test of MP01320-4

    Urine of six mice was measured. The mouse CD147 concentration of detected samples was determined to be 6,220.5 pg/mL with a range of 2,528.0 - 8372.5 pg/mL.

  • RAW264.7 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for mouse CD147, and measured 400.0 pg/mL.

    Sample test of MP01320-4

    RAW264.7 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for mouse CD147, and measured 400.0 pg/mL.

  • The mean CD147 concentration was determined to be 2,277.8 ng/mL in RAW264.7 cell extract based on a 4.8 mg/mL extract load.

    Sample test of MP01320-4

    The mean CD147 concentration was determined to be 2,277.8 ng/mL in RAW264.7 cell extract based on a 4.8 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01320-4 targets CD147 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: Mouse EMMPRIN/CD147 Recombinant antibody, PBS Only (Capture) 84488-5-PBS (241852B10). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse EMMPRIN/CD147 Recombinant antibody, PBS Only (Detector) 84488-4-PBS (241852H7). 100 μg. Concentration 1 mgl/ml.

Alternative CD147 matched antibody pairs: MP01320-1, MP01320-2, MP01320-3

Matched pair protein standard: Eg1530

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84488-5-PBS
Tested Reactivity mouse
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name basigin
Synonyms HT7 antigen, CD147, Bsg, Basigin, Basic immunoglobulin superfamily
GenBank accession numberNM_009768.2
Gene symbol CD147
UniprotP18572-1
Gene ID (NCBI) 12215
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84488-4-PBS
Tested Reactivity mouse
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name basigin
Synonyms HT7 antigen, CD147, Bsg, Basigin, Basic immunoglobulin superfamily
GenBank accession numberNM_009768.2
Gene symbol CD147
UniprotP18572-1
Gene ID (NCBI) 12215
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.