Mouse EMMPRIN/CD147 Recombinant antibody, PBS Only (Detector)

EMMPRIN/CD147 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241852H7

Cat no : 84488-4-PBS

Synonyms

HT7 antigen, CD147, Bsg, Basigin, Basic immunoglobulin superfamily

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01320-4

  Sandwich ELISA standard curve of MP01320-4, Mouse CD147 Recombinant Matched Antibody Pair - PBS only. 84488-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1530. 84488-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP01320-4

  Urine of six mice was measured. The mouse CD147 concentration of detected samples was determined to be 6,220.5 pg/mL with a range of 2,528.0 - 8372.5 pg/mL.

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  Sample test of MP01320-4

  RAW264.7 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for mouse CD147, and measured 400.0 pg/mL.

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  Sample test of MP01320-4

  The mean CD147 concentration was determined to be 2,277.8 ng/mL in RAW264.7 cell extract based on a 4.8 mg/mL extract load.

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  Sample test of MP01320-4

  Serum of six mice was measured. The mouse CD147 concentration of detected samples was determined to be 9,267.4 pg/mL with a range of 6,072.1 - 10,344.1 pg/mL.

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  Cytometric bead array standard curve of MP01320-1

  Cytometric bead array standard curve of MP01320-1, MOUSE EMMPRIN/CD147 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84488-2-PBS. Detection antibody: 84488-4-PBS. Standard: Eg1530. Range: 0.781-50 ng/mL.

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  Cytometric bead array standard curve of MP01320-3

  Cytometric bead array standard curve of MP01320-3, MOUSE EMMPRIN/CD147 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84488-3-PBS. Detection antibody: 84488-4-PBS. Standard: Eg1530. Range: 0.781-50 ng/mL.

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Product Information

84488-4-PBS targets EMMPRIN/CD147 as part of a matched antibody pair:

MP01320-1: 84488-2-PBS capture and 84488-4-PBS detection (validated in Cytometric bead array)

MP01320-3: 84488-3-PBS capture and 84488-4-PBS detection (validated in Cytometric bead array)

MP01320-4: 84488-5-PBS capture and 84488-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: basigin
• Calculated Molecular Weight: 42kDa
• GenBank Accession Number: NM_009768.2
• Gene Symbol: CD147
• Gene ID (NCBI): 12215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.